Micro-droplet digital PCR primer, probe, kit and method for quantitatively detecting ginseng

• Main Authors: LIN WEI, HUANG YONGHUI, SHAN ZHENJU, LIAO LI, LI WEIGANG, XU MIAOFENG
• Format: Patent
• Language: Chinese; English
• Published: 30.04.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the field of molecular biological detection, and discloses a micro-droplet digital PCR primer, a probe, a kit and a method for quantitatively detecting ginseng. The nucleotide sequence of the primer is shown as 5'-AGTTGCGCCCGAAGCCATTA-3' (SEQ ID NO.1), and the nucleotide sequence of the probe is shown as 5'-AGACACGGGAGGCCATTATC-3' (SEQ ID NO.2). The nucleotide sequence of the probe is shown as 5'-ATCACTCCCTTGCGGGAGTTGA-3' (SEQ ID NO.3). By adopting the specific primer and the probe, the ginseng can be quantitatively detected, and the specific primer and the probe have good specificity and sensitivity. 本发明属于分子生物学检测领域，公开了一种定量检测人参的微滴式数字PCR引物、探针、试剂盒及方法。所述引物的核苷酸序列如下所示：5'-AGTTGCGCCCGAAGCCATTA-3'(SEQ ID NO.1)，5'-AGACACGGGAGGCCATTATC-3'(SEQID NO.2)。所述探针的核苷酸序列如下所示：5'-ATCACTCCCTTGCGGGAGTTGA-3'(SEQ ID NO.3)。采用上述特异性引物和探针能够对人参进行定量检测，并具有良好的特异性和灵敏性。