Inulase mutant MutY119T with changed hot salt property and application thereof

The invention discloses an inulase mutant MutY119T with changed hot salinity and application thereof, the amino acid sequence of the inulase mutant MutY119T is shown as SEQ ID NO.1, and the amino acid sequence of the mutant MutY119T is obtained by mutating tyrosine at the 119th site of wild excision...

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Main Authors ZHANG RUI, HUANG ZUNXI, LI JUNJUN, CEN XIAOLONG, ZHOU JUNPEI, GAO YANXIU, HAN NANYU, XU BO, WU QIAN, TANG XIANGHUA
Format Patent
LanguageChinese
English
Published 30.04.2021
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses an inulase mutant MutY119T with changed hot salinity and application thereof, the amino acid sequence of the inulase mutant MutY119T is shown as SEQ ID NO.1, and the amino acid sequence of the mutant MutY119T is obtained by mutating tyrosine at the 119th site of wild excision inulase InuAMN8 into threonine. Compared with a wild enzyme InuAMN8, the mutant enzyme MutY119T has the advantages that the low-temperature activity is improved, the thermal stability is reduced, the activity and the stability in sodium chloride are reduced, the safe use of the enzyme is facilitated, and the mutant enzyme MutY119T is applied to the technical field of biology under the low-temperature environment requirement. The low-temperature excision inulase mutant MutY119T can be applied to the industries of food, wine brewing, washing and the like. 本发明公开了一种热盐性改变的菊粉酶突变体MutY119T及其应用，该菊粉酶突变体MutY119T的氨基酸序列如SEQ ID NO.1所示，该突变体MutY119T的氨基酸序列是将野生外切菊粉酶InuAMN8的第119位酪氨酸突变为苏氨酸获得的。与野生酶InuAMN8相比，本发明的突变酶MutY119T的低温活性提高，热稳定性
Bibliography:Application Number: CN202110041566