Probe set for efficiently painting sub-gene groups A and B of peanuts, design method and application

• Main Authors: HUANG BINGYAN, DONG WENZHAO, DU PEI, LIU HUA, ZHANG XINYOU, FU LIUYANG, WANG QIAN, ZHANG ZHONGXIN
• Format: Patent
• Language: Chinese; English
• Published: 27.04.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a probe set for efficiently painting sub-gene groups A and B of peanuts and design and application methods. The probe set for the sub-gene groups A and B of the peanuts is designed through a bioinformatics method by using genome sequence information of a peanut cultivated species Tifrunner, a technology for painting the sub-gene group A or B of the peanuts is established for the first time, and the technology can be used for painting peanut chromosome materials in batch size and efficiently identifying chromosome composition and chromosome translocation of the peanuts and wild species with close affiliation with the sub-gene groups. The established technology for painting the sub-gene group A or B of the peanuts can be used for identifying chromosomes of the sub-gene groups of the peanuts instead of fluorescence in-situ hybridization taking genomic DNA A.duranensis and A.ipaensis as probes; and the operating steps are simple, the cost is low, the consumed time is short, the efficiency