METHODS AND DEVICES FOR MIXING IN A MICROFLUIDIC SYSTEM

• Main Authors: KARUNAKARAN AATHAVAN, AGAH ALI, VENKATESAN MURALI, GHEISSARI PARGOL, BARNARD STEVEN, WANG JUNHUA, ABEILLE FABIEN, RIVAL ARNAUD, DEHLINGER DIETRICH, CIESLA CRAIG
• Format: Patent
• Language: Chinese; English
• Published: 16.04.2021
• Subjects: MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; TRANSPORTING

A method for reducing sequencing by synthesis cycle time using a microfluidic device is provided. The microfluidic device comprises a flow cell (20) having an inlet port, an outlet port, and a flow channel (21) extending between the inlet port and the outlet port, wherein the flow channel receives an analyte of interest and one or more reagents for analyzing and detecting molecules. To aid in the acceleration of the reactions, the microfluidic device comprises a mixing device to increase the rates of diffusion of the reagents from the fluid bulk to an active surface of the flow cell. The mixing device comprises at least one of an electrothermal mixing device, an active mechanical mixing device, and a vibrational mixing device. 提供了使用微流体装置减少合成测序循环时间的方法。微流体装置包括具有入口、出口和在入口与出口之间延伸的流动通道(21)的流动池(20)，其中流动通道接受感兴趣的分析物和用于分析和检测分子的一种或多种试剂。为了有助于加速反应，微流体装置包括混合装置以将试剂从流体主体扩散至流动池的活性表面的速率提高。混合装置包括电热混合装置、主动机械混合装置和振动混合装置中的至少一种。