Cancer gene methylation detection system and cancer in-vitro detection method performed in system

• Main Authors: LI YONGJUN, HAN XIAOLIANG, WU NINGNING, WANG JIANMING, GUO YUANYUAN
• Format: Patent
• Language: Chinese; English
• Published: 16.04.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Disclosed herein is a system for detecting cancer methylation. The system comprises a sample collection module for collecting a subject sample, a DNA extraction module used for extracting and purifying DNA in the sample, a library building module used for building a DNA library for sequencing for the purified DNA sample, a conversion module used for converting the constructed DNA library by using a bisulfite, a pre-PCR amplification module used for carrying out pre-PCR amplification on the DNA library subjected to bisulfite conversion, a hybrid capture module used for carrying out hybrid capture on the sample subjected to the pre-PCR amplification by utilizing a probe composition, a post-PCR amplification module used for amplifying a product captured by hybridization by utilizing PCR, a sequencing module used for carrying out high-throughput next-generation sequencing on a product subjected to hybridization capture after PCR amplification, a data analysis module used for analyzing the sequencing data and dete