NUCLEIC ACID APTAMERS TARGETING LYMPHOCYTE ACTIVATION GENE 3 (LAG-3) AND USES THEREOF

• Main Authors: ZHANG YIZHONG, CHIANG CHIEN-HAO, KAO YI-WEI
• Format: Patent
• Language: Chinese; English
• Published: 13.04.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

Nucleic acid aptamers capable of binding to lymphocyte activation gene 3 (LAG-3) and uses thereof for modulating immune responses. Such aptamers may comprise a G-rich motif, for example, GX1GGGX2GGTX3A (SEQ ID No: 1), in which each of X1 and X2 are independently G, C, or absent, and X3 is T or C, or L- (G)n-L', in which n is an integer of 5-9 inclusive, and L and L'are nucleotide segments having complementary sequences. Also provided herein are multimeric nucleic acid aptamers containing a backbone moiety, which comprises a palindromic sequence. 能够结合淋巴细胞活化基因3(LAG-3)的核酸适体及其用于调节免疫反应的用途。这种适体可包括富含G的基序，例如，GX1GGGX2GGTX3A(SEQ ID NO：1)，其中X1和X2各自独立地为G、C或不存在，以及X3为T或C，或L-(G)n-L'，其中n为5-9的整数(包含5和9)，且L和L'为具有互补序列的核苷酸区段。本文还提供了含有骨架部分的多聚体核酸适体，该骨架部分包括回文序列。