Method, fluorescent PCR primers and probe for detecting burkholderia gladioli and bongkrekic acid toxin-producing strain

• Main Authors: ZHANG YUE, HUANG JIANFEI, CHEN GUOPEI, WANG XIAOWEN, LI BIFANG, WANG YUANYANG, CHEN JING, YANG GUOWU
• Format: Patent
• Language: Chinese; English
• Published: 13.04.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a method, fluorescent PCR primers and probe for detecting burkholderia gladioli and a bongkrekic acid toxin-producing strain. The fluorescent PCR primers and probe for detecting the burkholderia gladioli include a PBA-F primer with a sequence as shown in SEQ ID NO:1, a PBA-R primer with a sequence as shown in SEQ ID NO:2, and a PBA fluorescent probe with a sequence as shown in SEQ ID NO:3. According to the technical scheme, the burkholderia gladioli and thebongkrekic acid toxin-producing strain are subjected to real-time fluorescent PCR detection by adopting a two-step method; the blank of molecular detection is filled; the method can be used for rapidly screening the burkholderia gladioli and the bongkrekic acid toxin-producing strain in foods; and the method is good in specificity, high in repeatability and high in sensitivity, can check hidden dangers in time and has relatively high practical value. 本发明提供了一种唐菖蒲伯克霍尔德氏菌及米酵菌酸产毒株的检测方法、检测用荧光PCR引物和探针，所述唐菖蒲伯克霍尔德氏菌检测用荧光PCR引物和探针包括PBA-F引物，序列如S