Method for monitoring wetland wild vertebrate diversity based on lake wetland eDNA extraction technology

• Main Authors: CAI WANG, WEN QINGZHONG, WU FUQIN, SONG JINGXIN, HUA CHAOLANG, YANG CHUNYAN
• Format: Patent
• Language: Chinese; English
• Published: 06.04.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a method for monitoring wetland wild vertebrate diversity based on a lake wetland eDNA extraction technology. The method comprises the following steps of (1) sample collection: taking a surface water sample of a lake wetland to be detected, (2) eDNA extraction: treating the taken water sample, and extracting eDNA, (3) a PCR process of the eDNA, (4) purifying and quantifying a PCR product of an eDNA technology, (5) high-throughput sequencing, (6) bioinformatics analysis, and (7) classifying and identifying, and determining the diversity composition of vertebrates in the lake wetland to be detected. According to the method, a surveyor does not need to have traditional biological recognition and identification experience, sampling is easy, detection is convenient, time and labor are saved, the cost is low, the detection efficiency is high, wetland organisms cannot be damaged, and wetland biodiversity protection is facilitated. 本发明公开了一种基于湖泊湿地eDNA提取技术监测湿地野生脊椎动物多样性的方法，包括如下步骤：(1)样品采集：取待测湖泊湿地的