Method for effectively reducing damage of nano ZnO particles to takifugu obscurus

• Main Authors: ZHANG JIANYUN, WANG JUN, HE SHUFENG, ZHANG HUI, TANG LEI, LI TING, CHEN QIUWEN
• Format: Patent
• Language: Chinese; English
• Published: 06.04.2021
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; FORESTRY; HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATIONTECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING ORPROCESSING OF MEDICAL OR HEALTHCARE DATA; HUMAN NECESSITIES; HUNTING; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; NEW BREEDS OF ANIMALS; PHYSICS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; TESTING; TRAPPING

The invention relates to a method for effectively reducing damage of nano ZnO particles to takifugu obscurus. The method is characterized by comprising the following steps of setting ZnO NPs with different concentrations and treatment groups with different salt concentrations in pairs by adopting a laboratory control variable experiment; putting juvenile takifugu obscurus into different treatment groups, feeding baits at regular time every day, cleaning dirt in time, and replacing a new treatment solution every day; conducting the experiment for 4 days, observing the survival condition of the juvenile fish every day, and taking gill, kidney and intestine tissues for physiological and biochemical detection on the last day; and analyzing and evaluating the effect of salinity on relieving the toxicity of ZnO NPs by combining the survival condition of the juvenile fish under each treatment, the half effect concentration EC50 of each time period, the physiological and biochemical indexes and the comprehensive biom