Preparation method of aligned carbon nanotube hydrogel film for myocardial cell culture

• Main Authors: CHI JUNJIE, ZHANG DAGAN, WANG YUETONG, CHEN ZHUOYUE, ZHAO YUANJIN
• Format: Patent
• Language: Chinese; English
• Published: 02.04.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR RELEVANT APPARATUS; TRANSPORTING; VINEGAR; WINE

The invention discloses a preparation method of an aligned carbon nanotube hydrogel film for myocardial cell culture, which comprises the following steps of: preparing an aligned carbon nanotube hydrogel mold, and injecting a hydrogel prepolymer into the hydrogel mold in the direction of aligned carbon nanotubes in a gap between two glass slides; curing the hydrogel precursor through ultraviolet irradiation; putting the hydrogel mold into ultrapure water, and separating the glass slides from the cured hydrogel to obtain an aligned carbon nanotube compounded hydrogel film; and paving a biocompatible thin layer on the surfaces of the aligned carbon nanotubes. The aligned carbon nanotube hydrogel film has good electrical conduction characteristics, can be used for culturing and detecting myocardial cells, enhances the conduction of electrical signals between the myocardial cells, and increases the consistency of the pacing rate of the myocardial cells; and the film has the advantages of reusability, low cost, ca