METHODS OF PRODUCING CELLS EXPRESSING A RECOMBINANT RECEPTOR, AND RELATED COMPOSITIONS

• Main Authors: VONG QUEENIE, NYE CHRISTOPHER HEATH, SATHER BLYTHE D, WELSTEAD G. GRANT, BORGES CHRISTOPHER, BURLEIGH STEPHEN MICHAEL
• Format: Patent
• Language: Chinese; English
• Published: 30.03.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

Provided are methods for engineering immune cells, cell compositions containing engineered immune cells, kits and articles of manufacture for targeting nucleic acid sequence encoding a recombinant receptor to a particular genomic locus and/or for modulating expression of the gene at the genomic locus, and applications thereof in connection with cancer immunotherapy comprising adoptive transfer ofengineered T cells. These may involve genetic disruption of at least one site within a TRAC gene and/or a TRBC gene and integration of the transgene encoding for the recombinant receptor at or near one of the at least one target site. 本文提供了用于工程化免疫细胞的方法、含有工程化的免疫细胞的细胞组合物、用于将编码重组受体的核酸序列靶向至特定基因组基因座和/或用于调节所述基因组基因座处基因的表达的试剂盒和制品、及其与包括工程化的T细胞的过继转移的癌症免疫疗法结合的应用。这些可以涉及TRAC基因和/或TRBC基因内的至少一个位点的遗传破坏以及编码所述重组受体的所述转基因在所述至少一个靶位点中的一个位点处或附近的整合。