HUMAN LIVER SCAFFOLDS

• Main Authors: DE COPPI PAOLO, MAZZA GIUSEPPE, MALAGO MASSIMO, PINZANI MASSIMO
• Format: Patent
• Language: Chinese; English
• Published: 26.03.2021
• Subjects: CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; HUMAN NECESSITIES; HYGIENE; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEDICAL OR VETERINARY SCIENCE; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL

This invention relates to methods for decellularising human liver tissue to produce human hepatic extracellular matrix (ECM) scaffolds, for example for use in therapy or disease modelling. The methodsinvolve mechanically damaging cells in the tissue, for example by freeze thaw, and then subjecting the liver tissue to multiple cycles of osmotic stress, detergent treatment and protease and/or DNAase treatment to produce a decellularised human ECM scaffold. 本发明涉及用于去细胞化人类肝脏组织以生产例如用于治疗或疾病建模中的人类肝脏细胞外基质(ECM)支架的方法。方法涉及，例如，通过冻融，然后，对肝脏组织施加多个循环的渗透应力，以机械方式破坏组织中的细胞，进行洗涤剂处理和蛋白酶和/或DNA酶处理，以生产去细胞化的人类ECM支架。