Lentiviral expression vector with high expression of neuroblastocyte miR-200b/c and construction method thereof

The invention discloses a lentiviral expression vector with high expression of neuroblastocyte miR-200 b/c. The lentiviral expression vector comprises a PCDH-copGFP vector and a miR200b/c gene sequence, wherein the miR200b/c gene sequence is inserted into the PCDH-copGFP vector through enzyme digest...

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Bibliographic Details
Main Authors LIU JIANJUN, NIE LULIN, REN XIAOHU, SUN QIAN
Format Patent
LanguageChinese
English
Published 19.03.2021
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a lentiviral expression vector with high expression of neuroblastocyte miR-200 b/c. The lentiviral expression vector comprises a PCDH-copGFP vector and a miR200b/c gene sequence, wherein the miR200b/c gene sequence is inserted into the PCDH-copGFP vector through enzyme digestion to obtain the lentiviral expression vector with high expression of miR200b/c; and precursor DNAsequences of the PCDH-copGFP vector and the miR200b/c both comprise two enzyme digestion sites of BamH1 and EcoRI. According to the lentiviral expression vector with high expression of neuroblastocytemiR-200b/c and a construction method thereof provided by the embodiment of the invention, the miR200b/c gene sequence is inserted into the PCDH-copGFP vector through enzyme digestion to obtain the lentiviral expression vector with high expression of miR200b/c, the transfection efficiency is high, and the expression of miRNA-200 b/c can be stably inhibited in neuroblastocytes, so that the gene expression is regulated and c
Bibliography:Application Number: CN202011256614