Method for immobilizing egg yolk membrane protein and application thereof

• Main Authors: ZHENG QIUSHA, YANG MUCHEN, WAN ZHIQIANG, ZHAO WENNENG, XIAO PENGCHENG, ZENG ZIHANG, AI MIN, LIU YONG, GAO XIONG, JI ZIJUAN, XIAO LONGSHENG, WANG HUAIXING
• Format: Patent
• Language: Chinese; English
• Published: 16.03.2021
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for immobilizing egg yolk membrane protein and application of the egg yolk membrane protein. The method for immobilizing egg yolk membrane protein comprises the following steps: treating an egg to obtain an egg yolk membrane, dyeing the egg yolk membrane by using a Golgi-Cox reagent, and blackening the egg yolk membrane by using LiOH. According to the method for observing the morphology of the egg yolk membrane and the membrane protein of the egg yolk membrane, the egg yolk membrane protein of the egg is fixed through the method, then the fixed egg yolk membrane is flatly laid on the carbon conductive adhesive to be naturally aired, stored in a dark place and sprayed with metal for electron microscope scanning analysis, and the morphology of the egg yolk membrane and the membrane protein of the egg yolk membrane can be observed. The Golgi-Cox staining method is used for immobilizing the egg yolk membrane and the membrane protein of the egg yolk membrane, mercury metal ions in