Universal rapid detection kit for mycoplasma hyopneumoniae antigen content and application of detection kit

• Main Authors: XIONG QIYAN, WANG LI, HAN RUI, LIU BEIBEI, WANG JIA, WEI YANNA, YU YANFEI, HAO FEI, SHAO GUOQING, FENG ZHIXIN
• Format: Patent
• Language: Chinese; English
• Published: 12.03.2021
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a universal rapid detection kit for mycoplasma hyopneumoniae antigen content and application of the detection kit, and belongs to the technical field of bioengineering. The kitcomprises an elisa plate and rabbit anti-EF-Tu serum, wherein the elisa plate is coated with a mycoplasma suis antigen; the rabbit anti-EF-Tu serum is obtained by immunizing New Zealand rabbits with recombinant EF-Tu protein and collecting blood and separating serum, and the sequence of the recombinant EF-Tu protein is shown as SEQ ID NO: 4. The kit disclosed by the invention is short in time consumption and high in accuracy, and can be used for detecting the content of the mycoplasma hyopneumoniae antigen in the inactivated vaccine. 本发明提供一种通用的猪支原体抗原含量快速检测试剂盒及其应用，属于生物工程技术领域。该试剂盒包括采用猪支原体抗原包被的酶标板和兔抗EF-Tu血清；所述兔抗EF-Tu血清是采用重组EF-Tu蛋白免疫新西兰兔，采血分离血清后所得，所述重组EF-Tu蛋白的序列如SEQ ID NO:4所示。本发明试剂盒，该方法耗时短、准确性高、能检测灭活疫苗中猪支原体抗原含量。