Photoinduction-activated Dre recombination system

• Main Authors: LI DALI, WU YINGYIN, LI HUIYING, LIU MINGYAO
• Format: Patent
• Language: Chinese; English
• Published: 23.02.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a photoinduction-activated Dre recombination system, and particularly provides a split protein and a fusion protein. The photoinduction-activated Dre recombination system has higher space-time specificity, can be applied to multiple fields of lineage tracing, cell elimination and the like, is combined with the specificity of a promoter, and realizes more accurate gene operation by utilizing local blue light irradiation. 本发明提供了一种光诱导激活的Dre重组系统。具体地，本发明提供了一种拆分式蛋白以及融合蛋白，本发明的光诱导激活的Dre重组系统具有更高的时空特异性，可应用于谱系示踪、细胞清除等多个领域，联合启动子的特异性，利用局部蓝光照射实现更为精确的基因操作。