Method for detecting three active ingredients of thunberg fritillary bulb in biological sample based on solid phase extraction-liquid chromatography-mass spectrometry

• Main Authors: YING JIAMIN, TANG CHUNLAN, CHU DONGHUI
• Format: Patent
• Language: Chinese; English
• Published: 29.01.2021
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for detecting three active ingredients of thunberg fritillary bulb in a biological sample based on solid-phase extraction liquid chromatography-mass spectrometry. Thedetection method relates to application of analytical chemistry in biomedicine. The method comprises the following specific steps of: taking a biological sample, adding the biological sample into a pre-activated MCX solid-phase extraction column, washing the biological sample with water and methanol to remove impurities, eluting the sample with an ammonia water-methanol solution, and carrying outvacuum centrifugal concentration and drying on the eluent; and concentrating, re-dissolving with methanol, filtering, and making analysis with a liquid chromatography-mass spectrometry instrument. According to the detection method, solid-phase extraction is combined with a high-resolution instrument, the sensitivity is high, interference is little, the three active ingredients of thunberg fritillary bulb in the biological