Mutant of AroG and application of mutant in amino acid producing genetically engineered bacteria

• Main Authors: DING DONGQIN, ZHU YARU, ZHANG DAWEI, BAI DANYANG
• Format: Patent
• Language: Chinese; English
• Published: 26.01.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a mutant of AroG and application of the mutant in amino acid producing genetically engineered bacteria, and discloses application of inactivation of the mutant of 3-deoxy-alpha-arabinoheptanoic acid-7-phosphate synthase AroG and a regulatory factor RpoS in construction of tryptophan producing strains. When the genetically engineered bacterium obtained by the invention is used for fermentation, effective accumulation of L-tryptophan can be obtained, the yield of the L-tryptophan can be obviously improved, and the genetically engineered bacterium has an industrial application value. 本发明AroG的突变体及其在产氨基酸基因工程菌中的应用，公开了一种3-脱氧-α-阿拉伯庚酮糖酸-7-磷酸合酶AroG的突变体和调控因子RpoS的失活在产色氨酸菌株的构建中的应用。利用本发明获得的基因工程菌进行发酵，可以获得L-色氨酸的有效积累，能够明显提高其产量，具有产业化应用价值。