HLA-G full-length protein as well as expression vector, expression engineering strain and preparation method thereof

The invention provides an HLA-G full-length protein as well as an expression vector, an expression engineering strain and a preparation method thereof. A nucleotide sequence of an expression region ofthe expression vector includes a nucleotide sequence as shown in SEQ ID NO.3, and the engineering st...

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Bibliographic Details
Main Authors WANG YING, YIN TINGZI, YUAN WENWEN, YI WANGXUE, YU QIAN, KE HONG
Format Patent
LanguageChinese
English
Published 19.01.2021
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention provides an HLA-G full-length protein as well as an expression vector, an expression engineering strain and a preparation method thereof. A nucleotide sequence of an expression region ofthe expression vector includes a nucleotide sequence as shown in SEQ ID NO.3, and the engineering strain contains the HLA-G full-length protein expression vector. The HLA-G full-length protein is prepared by induction expression and purification on the HLA-G full-length protein expression engineering strain. According to the invention, an optimized escherichia coli expression system is adopted toexpress the human HLA-G full-length protein in vitro, so that high-yield and high-purity expression of the human HLA-G full-length protein is realized. The human HLA-G full-length protein provided bythe invention contains a transmembrane region and has a structure similar to that of natural protein verified by ELISA, and the human HLA-G full-length protein with high yield, high purity and structural activity can be prepar
Bibliography:Application Number: CN202011129616