Primer probe combination, kit and method for detecting novel coronavirus

• Main Authors: LI YANGXIA, WANG XIYING, LYU TANGSHAN, JIA JUNLING
• Format: Patent
• Language: Chinese; English
• Published: 15.01.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a primer probe combination, a kit and a method for detecting a novel coronavirus. The primer probe combination comprises primers for amplifying ORF 1ab genes, E genes and N genes of the novel coronavirus and corresponding probes for detecting the ORF 1ab genes, the E genes and the N genes of the novel coronavirus. According to the primer probe combination, an ORF 1ab gene, an E gene and an N gene of a novel coronavirus are used as detection targets; primer probe combination design is carried out on a segment with good conservative property of the ORF 1ab gene to the novel coronavirus, so that the specificity and the accuracy of detection can be ensured, and meanwhile, primer probe design of E gene and N gene segments is also added, so that the probability of missingdetection can be effectively reduced. In addition, due to the design of adding the human-derived internal standard, the sampling, extracting and detecting processes can be monitored, and a false positive result caused by unqu