Construction method of animal model of rat with compressive injury of nerve roots
The invention discloses a construction method of an animal model of a rat with compressive injury of nerve roots. The construction method comprises the following steps: (1) preparing a right-angled triangular rubber block; (2) completely exposing the nerve root L5 of the rat through an operation; (3...
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Main Authors | , , , , |
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Format | Patent |
Language | Chinese English |
Published |
15.01.2021
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses a construction method of an animal model of a rat with compressive injury of nerve roots. The construction method comprises the following steps: (1) preparing a right-angled triangular rubber block; (2) completely exposing the nerve root L5 of the rat through an operation; (3) inserting the rubber block into the spinal canal and pressing the nerve root L5; (4) sewing the cuts layer by layer to fix the rubber block until the rubber block cannot be inserted; (5) normally feeding rats, regularly observing histological change conditions of dorsal root ganglion and apoptosisconditions of dorsal root ganglion of the rat, detecting activity conditions of caspase-3, and observing protein expression conditions of cytokines. According to the construction method, the histopathology, cell apoptosis condition, activity condition of caspase-3 and expression condition of various cell factors of the animal model of the rat with compressive injury of nerve roots constructed bythe method are all similar |
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Bibliography: | Application Number: CN202010713984 |