Construction method of animal model of rat with compressive injury of nerve roots

The invention discloses a construction method of an animal model of a rat with compressive injury of nerve roots. The construction method comprises the following steps: (1) preparing a right-angled triangular rubber block; (2) completely exposing the nerve root L5 of the rat through an operation; (3...

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Bibliographic Details
Main Authors LIN HUANAN, XU LEQIN, ZHOU RUIJUAN, LU XIAOHUI, CHEN HAIPENG
Format Patent
LanguageChinese
English
Published 15.01.2021
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
CARE OF BIRDS, FISHES, INSECTS
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
HYGIENE
MEDICAL OR VETERINARY SCIENCE
NEW BREEDS OF ANIMALS
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR
TRAPPING
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Summary:The invention discloses a construction method of an animal model of a rat with compressive injury of nerve roots. The construction method comprises the following steps: (1) preparing a right-angled triangular rubber block; (2) completely exposing the nerve root L5 of the rat through an operation; (3) inserting the rubber block into the spinal canal and pressing the nerve root L5; (4) sewing the cuts layer by layer to fix the rubber block until the rubber block cannot be inserted; (5) normally feeding rats, regularly observing histological change conditions of dorsal root ganglion and apoptosisconditions of dorsal root ganglion of the rat, detecting activity conditions of caspase-3, and observing protein expression conditions of cytokines. According to the construction method, the histopathology, cell apoptosis condition, activity condition of caspase-3 and expression condition of various cell factors of the animal model of the rat with compressive injury of nerve roots constructed bythe method are all similar
Bibliography:Application Number: CN202010713984