Novel melittin and application thereof

• Main Authors: WEN XINXIN, MO XIANGGUI, TAN KEN, MENG YICHUAN
• Format: Patent
• Language: Chinese; English
• Published: 08.01.2021
• Subjects: CHEMISTRY; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES

The invention discloses a novel melittin and an application thereof. The amino acid sequence of the melittin is INWKGIAAMAKRLL; the synthetic method of the melittin specifically comprises the following steps of extracting Vespa velutina poison sac RNA, carrying out reverse transcription PCR to construct a cDNA library, screening out a target gene by using a special primer, and synthesizing to obtain the melittin. The melittin VV-MP-3 disclosed by the invention has relatively high inhibitory activity on gram-positive bacteria, and tests prove that the melittin VV-MP-3 has a relatively good bacteriostatic effect on staphylococcus aureus and escherichia coli, and the bacteriostatic rates are 0.998 +/-0.003 and 1.000 +/-0.001 respectively; the melittin VV-MP-3 also has an inhibiting effect ongram-negative bacillus subtilis, and can be used for preparing antibacterial drugs or medical consumables; and the melittin VV-MP-3 disclosed by the invention has remarkable antioxidant performance, has a certain inhibitory e