Kit for detecting porcine pseudorabies virus (PRV) by combining centrifugal micro-fluidic chip with loop-mediated isothermal amplification (LAMP) technology

• Main Authors: LIU YANHONG, LAN TIAN, MA JINGYUN, SUN YUAN, ZHOU LING, CHEN YONGHUI, LI QIANNIU, LU XIANDONG
• Format: Patent
• Language: Chinese; English
• Published: 05.01.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of molecular detection, discloses a kit for detecting porcine pseudorabies virus (PRV) by combining a centrifugal micro-fluidic chip with a loop-mediated isothermal amplification (LAMP) technology, and establishes a PRV nucleic acid detection method which is rapid, sensitive, high in accuracy and high in repeatability by combining the micro-fluidic chiptechnology. The micro-fluidic chip detection method is high in specificity, and the lowest detection limit can reach 10 copies/[mu]L. According to the micro-fluidic chip kit for detecting porcine pseudorabies virus (PRV), a chip analysis device is small and portable, and the defects of time consumption, labor consumption and high cost of PRV detection can be overcome, so that the detection sensitivity and specificity are improved, the labor and equipment cost is reduced, the detection period is shortened, and the requirements of field detection are met. The rapid detection technology can bepopularized and applied to