Preservation method for prolonging survival time of frozen and thawed sperms

• Main Authors: RU ZHENYUAN, ZHANG XIANGDONG, LIU CHUANJIANG, ZHANG YUNHAI, GUO TENGLONG, QI DEZHOU, CAO ZUBING, YANG XIANSHU
• Format: Patent
• Language: Chinese; English
• Published: 05.01.2021
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; TRAPPING

The invention discloses a preservation method for prolonging the survival time of frozen and thawed sperms, which specifically comprises the following steps: preparing thawing liquid, thawing the frozen sperms by utilizing the thawing liquid, filling a sperm collecting bottle with the thawed frozen sperms, discharging excess air in the sperm collecting bottle, putting the sperm collecting bottle into a vacuum cup filled with water at 17 DEG C, and preserving the frozen sperms by screwing a vacuum cup cap. The preservation method for prolonging the survival time of the frozen and thawed spermscan ensure that the vitality of the frozen and thawed sperms can still be kept like the vitality of the frozen and thawed sperms which are just unfrozen after the frozen and thawed sperms are stored for 24 hours, and the mating requirements of female animals can be completely met. 本发明公开了一种延长冻融精子存活时间的保存方法，所述方法具体包括以下步骤：配制解冻液，利用解冻液将冷冻精子解冻，将解冻后的冷冻精液装入集精瓶中，排去集精瓶中多余空气后，将集精瓶置于装有17℃水的保温杯中，旋紧保温杯瓶盖即可进行保存。该延长冻融精子存活时间的保存方法可以保证冻融精子储存2