Method for detecting content of nucleoside in polyporus umbellatus

• Main Authors: HUA MOLI, XU YUNHUI, LIU JIE, ZHOU JING, ZHU MINHANG, ZHANG QIANQIAN
• Format: Patent
• Language: Chinese; English
• Published: 29.12.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for detecting the content of nucleoside in polyporus umbellatus. The detection method comprises the following steps of: using geniposide as an internal standard, and detecting the nucleoside in a polyporus umbellatus test solution by adopting high performance liquid chromatography. According to the method, the nucleoside component is used as a quality control itemof polyporus umbellatus standard decoction and formula particles, and the internal standard substance is combined, so that the content of the nucleoside component contained in the polyporus umbellatuscan be well determined. The detection method is simple and stable to operate, good in reproducibility and high in detection efficiency, and has relatively high practicability. 本发明公开了一种猪苓中核苷含量的检测方法。该检测方法包括下述步骤：以栀子苷为内标，采用高效液相色谱检测猪苓供试品溶液中的核苷即可。本发明中将核苷类成分作为猪苓标准汤剂和配方颗粒的质量控制项，结合内标物，可较好实现对其中所含核苷类成分的含量测定。本发明的检测方法操作简单、稳定、重现性好、检测效率高，具有较强实用性。