High-density culture method of lactobacillus helveticus, and preparation and application of high-activity bacterial powder

• Main Authors: CHEN LI'E, WENG LUWEI, ZHAO YUE, REN XUELIANG, CHEN CAILING, CHEN ZUOGUO, LIN GUODONG, LI YANJUN, LI LI, ZHENG ZHIYAO, CHEN SU
• Format: Patent
• Language: Chinese; English
• Published: 25.12.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of microbial culture and application, and discloses a high-density culture method of lactobacillus helveticus, and preparation and application of high-activity bacterial powder, aiming at the problems of low activity and long culture period of lactic acid bacteria in the prior art. The high-density culture method comprises the following steps of (1) inoculating a lactobacillus helveticus strain into a seed culture medium for activation, performing culturing to obtain a seed solution; and (2) inoculating the seed solution into a lactobacillus helveticus high-density fermentation culture medium, and carrying out fermentation culture at 35-43 DEG C under an anaerobic stirring condition to obtain the viable bacteria fermentation liquor after the fermentation is finished. The fermentation liquor obtained by the method is applied to preparation of high-activity lactobacillus helveticus powder. A high-density whey proliferation culture medium is provided, and lactobacillu