High-throughput detection method for content of ergothioneine in to-be-detected sample and application of high-throughput detection method

• Main Authors: ZHENG QIANWANG, GUO LIQIONG, LIN JUNFANG, YE ZHIWEI, YOU JUNJIAN
• Format: Patent
• Language: Chinese; English
• Published: 22.12.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses a high-throughput detection method for the content of ergothioneine in a to-be-detected sample and application of the high-throughput detection method, and belongs to the technical field of microbial breeding. The method comprises the following steps: adjusting the pH value of a to-be-detected sample to 1.9-2.0, firstly adding a ferric thiocyanate solution into an elisa plate, then adding the to-be-detected sample, and conducting blowing, beating and mixing; and recording the absorbance by using a microplate reader, wherein the wavelength used by the microplate reader is 450nm-470nm. The lower the absorbance is, the higher the ergothioneine content is. The method has the characteristics of high efficiency, rapidness, convenience and low cost, and is suitable for research activities which do not require accurate determination of the ergothioneine content of each sample; and the defects of time consumption and high cost of an LCMS detection method are overcome. 本发明公开一种用于待测样品间麦角硫因含量高低的高通量