Animal model for protecting high-oxygen exposed lung and brain injury by umbilical cord mesenchymal stem cells

• Main Authors: DING MINGXING, LIN YIPING, YAO JINGCHAN
• Format: Patent
• Language: Chinese; English
• Published: 08.12.2020
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

The invention belongs to the technical field of models for science, medicines or mathematics, and discloses an animal model for protecting high-oxygen exposed lung and brain injury by umbilical cord mesenchymal stem cells. Continuous high-oxygen is adopted to induce lung and brain injury in a rat model, and besides, lentiviral vector mediated green fluorescent protein labeled human umbilical cordblood mesenchymal stem cells are constructed. Trachea, nasal cavity and vein transplantation is adopted, and UC-MSc field planting, growth tracking and apoptosis detection, parallel histological evaluation, oxidative stress and inflammatory factor detection and function evaluation are performed. According to the invention, a lentiviral vector carrying GFP is used for transfecting UC-MSc, and threetransplantation methods of trachea, nasal cavity and caudal vein injection of the UC-MSc are adopted for comparison. Transplanting trachea, nasal cavity and caudal vein injection UC-MSc is performed,field planting conditions