In-vitro culture method for accelerating dendritic cell maturation and application thereof

• Main Authors: WANG GANG, YE YONGQING, ZHENG QINGFU
• Format: Patent
• Language: Chinese; English
• Published: 04.12.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of in-vitro cell culture, and particularly relates to an in-vitro culture method for accelerating dendritic cell maturation and application thereof. The method comprises the following steps: (1) resuspending PBMC cells in a culture container with a 1640 culture solution, and then placing the culture container in an incubator with constant temperature and a gas carbon source for culture; (2) shaking the culture container after culturing for a predetermined time, discarding suspended cells, then adding a DC serum-free medium, a GM-CSF reagent, an Il-4reagent and a cholesterol reagent, and continuing culturing in the incubator; and (3) adding LPS and IL-1 alpha when culturing for the third day, and continuing culturing until mature DC cells are obtained. The method can significantly promote the maturation of dendritic cells and shorten the culture time of the dendritic cells, and avoids the application of serum during the culture process, so that the clinical applicat