Application of toad venom polypeptide as toad sterene penetration enhancer

• Main Authors: ZHOU JING, MA HONGYUE
• Format: Patent
• Language: Chinese; English
• Published: 01.12.2020
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

The invention discloses application of toad venom polypeptide as a medicine penetration enhancer. The toad venom polypeptide is selected from KAGFAGDDAPR, RVAPEEHPVL, NDEELNKLLG, MLANEDYASLTKGIQNL, APAVLKE, KPQGASFT, FNPFR and ATVSARN. According to the invention, functional polypeptide capable of promoting permeation of toad sterenes and other drugs is screened out through a large number of experimental screening and an in-vitro Franz diffusion pool. Screening experiments show that the FNPFR and other toad venom polypeptides have a remarkable permeation enhancing effect on resibufogenin, cinobufagin, Cinobufotalin std. and other free or combined toad sterenes in guinea pig skin, drug absorption can be promoted, and a very good technical effect is achieved. 本发明公开了蟾酥多肽在作为药物促渗剂中的应用；所述的蟾酥多肽为KAGFAGDDAPR、RVAPEEHPVL、NDEELNKLLG、MLANEDYASLTKGIQNL、APAVLKE、KPQGASFT、FNPFR、ATVSARN。本发明通过大量实验筛选，通过体外Franz扩散池筛选出具有促进蟾蜍甾烯等药物促渗的功能多肽。本发明筛选实验表明：FNPFR等蟾酥多肽对豚鼠皮肤中脂蟾毒配基、华蟾酥毒基、华蟾毒它灵等游离型或结合型蟾蜍甾烯具有显著的促渗作用，可以促进药物吸收，取得了很好的技术效果。