Human follicle-stimulating hormone and in-vitro reassembly method thereof

• Main Authors: LIU JIANXI, HU JIANHONG, LI QINGWANG, HUA RONGMAO
• Format: Patent
• Language: Chinese; English
• Published: 27.11.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses a human follicle-stimulating hormone and an in-vitro reassembling method thereof, and belongs to the technical field of biological medicines and gene engineering. The human follicle-stimulating hormone provided by the invention is obtained by respectively expressing FSH alpha and FSH beta subunit genes in animal milk and then performing reassembling in vitro, and has the same biological function as Gonal-F. The preparation method comprises the following steps: constructing human FSH alpha and FSH beta gene expression vectors, expressing human FSH alpha and FSH beta subunits in the animal milk, and performing reassembling in vitro to obtain the human follicle-stimulating hormone with biological activity. The method can avoid accumulation of endogenous FSH with biological activity in an animal body, so that the risk that the animal suffers from tumors due to the accumulation of a large amount of the FSH with biological activity is reduced. 本发明公开了一种人促卵泡激素及其体外重组装方法，属于生物医药与基因工程技术领域。本发明提供的人促