Linker for preparing MGI/BGI platform NGS library and application of linker

• Main Authors: ZHOU QIHAO, CAO ZHEN, SONG DONGLIANG, WANG JIATING, LI ZHENZHEN
• Format: Patent
• Language: Chinese; English
• Published: 24.11.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a linker for preparing an MGI/BGI platform NGS library. The linker is formed by partial complementary pairing of a DNA single chain with a sequence shown in SEQ NO. 1 and a DNAsingle chain with a sequence shown in SEQ NO. 2. The invention also discloses an amplification primer combination corresponding to the linker. The amplification primer combination comprises an upstream primer with a sequence shown in SEQ NO. 3 and a downstream primer with a sequence shown in SEQ NO. 4. Compared with the prior art, the novel linker has the advantages of high effective connection efficiency, high library building yield, low preparation cost and high discrimination of linker connection product fragments, and is convenient for analysis and evaluation of connection effects. 本发明公开了一种可用于MGI/BGI平台NGS文库制备的接头，其由具有如SEQ NO.1所示序列的DNA单链和具有如SEQ NO.2所示序列的DNA单链部分互补配对而成。还公开了该接头所对应的扩增引物组合，包括具有如SEQ NO.3所示序列的上游引物和具有如SEQ NO.4所示序列的下游引物。与现有技术相比，本发明的新型接头具有有效连接效率高、建库产量高、制备成本较低、接头连接产物片段区分度高的优点，便于连接效果的分析与评估。