Test strip for detecting African swine fever virus serum antibody and application thereof

• Main Authors: LI YUE, XIONG QIYAN, ZHANG YUE, WANG LI, ZHANG LEI, LI JIAHAO, LU YUNAN, WEI YANNA, SHAO GUOQING, CHEN RONG, BAI YUN, HUA LIZHONG, LIU FEI, LIU BEIBEI, FENG ZHIXIN, XIE QINGYUN, SHAN YANKE
• Format: Patent
• Language: Chinese; English
• Published: 10.11.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a test strip for detecting an African swine fever virus serum antibody and application of the test strip, and belongs to the field of production and technology of veterinary biological diagnosis products. The test strip comprises a bottom plate, wherein a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are sequentially arranged on the bottom plate; the combination pad is coated with SPA marked by quantum dot microspheres; a first detection line and a quality control line are arranged on the nitrocellulose membrane, the first detectionline is coated with recombinant protein CD2v and recombinant protein MGF360, and the first detection line is arranged close to one side of the combination pad; the quality control line is coated witha chicken anti-SPA polyclonal antibody, and the quality control line is arranged close to one side of the water absorption pad. A sample diluent used in cooperation with the test strip is a boric acidbuffer solution containing