LC-MS (Liquid Chromatography-Mass Spectrometry) determination method for doxycycline residues in eggs

• Main Authors: XU PIAOPIAO, ZHANG MEI, ZHU LISHA, XIANG WENTING, ZENG JING, PENG DAPENG, OU YAHONG
• Format: Patent
• Language: Chinese; English
• Published: 03.11.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses an LCMS (Liquid Chromatography Mass Spectrometry) determination method for doxycycline residues in eggs, which comprises the steps of sample extraction and purification, preparation of a standard working solution, determination, result calculation and the like,wherein the chromatographic column is C18, the mobile phase A is a 0.05-0.5% formic acid aqueous solution, and themobile phase B is an acetonitrile solution; performing determination by adopting an ESI ion source, positive ion mode scanning and multi-ion reaction monitoring mode determination, wherein the mass-to-charge ratio of doxycycline parent ions is 445.0, the mass-to-charge ratios of daughter ions detected by secondary mass spectrometry are 154 and 428, and corresponding collision voltages are 37 eV and 29 eV respectively. According to the method, the average recovery rate is 78.51%-108.82%, the average relative standard deviation (RSD) is 5.96%-11.07%, the detection limit is only 0.5 mu g/kg, thequantification limit is on