Signal amplification magnetic bead technology system for nucleic acid detection based on the CRISPR technology and application thereof

• Main Author: ZHENG DUNWU
• Format: Patent
• Language: Chinese; English
• Published: 30.10.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a report magnetic bead. The report magnetic bead comprises a magnetic bead, nucleotide and an enzyme with high catalytic activity which are linked. The invention further discloses a signal amplification magnetic bead technical system for nucleic acid detection based on the CRISPR technology, and a construction method and application of the report magnetic bead. By introducing the report magnetic bead, the sensitivity of the signal amplification magnetic bead technology system is improved from the aM (1000-10000 copies/mL) concentration level to the zM (1-10 copies/mL) concentration level. 本发明公开了一种报告磁珠，所述报告磁珠包括磁珠、核苷酸和高催化活性的酶相链接。本发明还公开了基于CRISPR技术的核酸检测的信号放大磁珠技术系统，本发明还公开报告磁珠的构建方法以及应用。本发明通过引入报告磁珠，将信号放大磁珠技术系统的灵敏度从aM（1000-10000 copies/mL）浓度级别提高到zM（1-10 copies/mL）浓度级别。