Compositions and methods for unidirectional nucleic acid sequencing

• Main Authors: FULLER CARL, DAVIS RANDALL
• Format: Patent
• Language: Chinese; English
• Published: 27.10.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide. 本公开提供了用于对核酸样品进行测序的芯片、系统和方法。将标记的核苷酸提供至在膜中包含纳米孔的反应室中。所述标记的核苷酸的单个标记的核苷酸可以含有与核苷酸偶联的标签，所述标签可借助纳米孔检测。接下来，可以将标记的核苷酸的单个标记的核苷酸掺入与源自核酸样品的单链核酸分子互补的生长中的链中。借助纳米孔，在掺入单个标记的核苷酸后，可以检测与单个标记的核苷酸缔合的标签。当标签从核苷酸释放时，可以借助纳米孔来检测标签。