Fusion gene for regulating and controlling color of cotton fibers and expression vector and application of fusion gene

• Main Authors: XIAO YUEHUA, LI YAOHUA, MO TONG, LUO MING, RAN LINGFANG, ZENG JIANYAN, WANG YI, LIANG AIMIN
• Format: Patent
• Language: Chinese; English
• Published: 27.10.2020
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention provides a fusion gene for regulating and controlling color of cotton fibers and an expression vector and application of the fusion gene, and relates to the technical field of transgenicbreeding of plants. A 2A peptide capable of being self-cracked into small-fragment peptides is utilized, Lc and GhPAP1 genes are synthesized into the fusion gene, a fiber secondary wall specific promoter pFbl2A is adopted to start excessive expression of the fusion gene in the cotton fibers, and the anthocyanin content of the obtained transgenic cotton fibers is remarkably increased compared withthat of white fibers; the total anthocyanin content in the transgenic fiber is increased by 4093 times compared with that in the white fiber, so that the transgenic cotton generates purple red fibers, and the color range of the mature fibers is obviously expanded. 本发明提供了一种调控棉花纤维呈色的融合基因及其表达载体和应用，涉及植物转基因育种技术领域。本发明利用一种可以自裂解为小片段肽的2A肽，将Lc和GhPAP1基因组合成融合基因，采用纤维次生壁特异启动子pFbl2A启动所述融合基因在棉纤维中超量表达，得到的转基因棉纤维花青素含量较白色纤维显著提高，转基因纤维中总花青素量比白