Method for establishing completely Freund's adjuvant induced frozen shoulder animal model

• Main Authors: CHEN GANG, FAN GUOMING, HUANG CHENGLONG, CAI ZHENHAI, PAN JIE'EN
• Format: Patent
• Language: Chinese; English
• Published: 23.10.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for establishing a completely Freund's adjuvant induced frozen shoulder animal model. The method comprises the following steps that step S1, firstly, hair in a presetarea on the outer side of the right shoulder of an animal is cut off through bent shears; an animal is made to prostrate, and two hind limbs and left fore limb are fixed on a table; the skin around the injection part is disinfected by 75% alcohol; 50 [mu]L of CFA is injected, a complete Freund's adjuvant is injected into a single shoulder joint cavity to induce shoulder arthritis, a control animalis similarly injected with sterile normal saline, the day of first injection of CFA is marked as the first day of an experiment, and CFA is injected on the first day, the fourth day, the seventh dayand the thirteenth day respectively. The method is helpful to solve defects of the existing animal model for inducing ankle arthritis, and successfully establishes a shoulder joint pain model with aninflammatory basis. 本发明公开了一种