Mycoplasma bovis Mbov_0274 gene mutant and application thereof

• Main Authors: EHSANULLAH HIRANI, CHEN JIANGUO, CHEN YINGYU, ZHANG HUI, HU CHANGMIN, ZHAO GANG, GUO AIZHEN, CHEN XI
• Format: Patent
• Language: Chinese; English
• Published: 16.10.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses a mycoplasma bovis Mbov_0274 gene mutant, and belongs to the technical field of animal borne disease prevention and treatment. The mutant is named as mycoplasma bovis T9.202, is preserved in China Center for Type Culture Collection with the preservation No. of CCTCC NO:M 2020084, and the nucleotide sequence of the Mbov_0274 gene is shown as SEQID NO:1. The capacity of a T9.202 strain for stimulating expression of IL-8 by BoMac cells is detected by qRT-PCR, results show that the capacity of the mycoplasma bovis bacterial strain T9.202 for inducing the expression of cytokine by the BoMac cells is reduced, and the mycoplasma bovis Mbov_0274 gene mutant is hopeful to development of important effects in the field of mycoplasma bovis immunization control. 本发明公开了一种牛支原体Mbov_0274基因突变株，属于动物传染病防治技术领域，所述突变株命名为牛支原体(Mycoplasma bovis)T9.202，保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO:M 2020084，所述Mbov_0274基因的核苷酸序列为SEQ ID NO:1所示。本发明通过qRT-PCR检测T9.202株刺激BoMac细胞表达IL-8的能力，结果显示牛支原体菌株T9.202诱导BoMac细胞表达细胞因子的能力下降，可望在牛支原体免疫防治领