Paclitaxel synthase TcTS2, coding nucleotide sequence and application thereof

• Main Authors: HUANG SANWEN, LIANG HAIFEI, GOU JUNBO, XIONG XINGYAO, YAN JIANBIN, LI CHONG, DU RAN, LIAO QINGGANG
• Format: Patent
• Language: Chinese; English
• Published: 09.10.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a paclitaxel synthase TcTS2, a coding nucleotide sequence and application thereof, and relates to the technical field of plant genetic engineering. The amino acid sequence of the TcTS2 comprises or consists of the following sequences: a) an amino acid sequence shown as SEQ ID NO.1; or b) a functional homologous sequence having at least 80% sequence similarity with the aminoacid sequence shown as SEQ ID NO.1; or c) an amino acid sequence which is obtained by adding, deleting and replacing one or more amino acids in the amino acid sequence shown as SEQ ID NO.1 and has TcTS2 activity. TcTS2 and the nucleotide sequence for encoding TcTS2 provide a new gene resource for increasing the yield of paclitaxel, can be used for modifying chassis hosts, producing paclitaxel andintermediates thereof and the like in plant genetic engineering and metabolic engineering strategies, and have remarkable economic and social values. 本发明提供了一种紫杉二烯合酶TcTS2，编码核苷酸序列及其应用，涉及植物基因工程技术领域。TcTS2的氨基酸序列包含或由如下序列组成：a)SEQ ID