Multiplex amplification system for mouse short tandem repeats, and detection kit

• Main Authors: CHEN YAXIN, HUANG WEIYI, JIANG WANTING, LIANG XUANYI, QIN QINGTIAN
• Format: Patent
• Language: Chinese; English
• Published: 29.09.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a multiplex amplification system for mouse short tandem repeats. The multiplex amplification system comprises 18 pairs of primers, and 18 mouse STR sites can be amplified at the same time. The invention also provides a detection kit formed by the multiplex amplification system. The multiplex amplification system can obtain a great amount of information through once amplification. An amplification product has fluorescence, and can be directly detected on a sequencer. The result is accurate; the repeatability is high; a standard reference database can be built by the data; the operation is simple and convenient; short time is required; and scale production and automation can be easily realized. 本发明公开了一种小鼠短串联重复序列的多重扩增体系，该多重扩增体系中包含18对引物，可同时扩增18个小鼠的STR位点。本发明还包括由所述多重扩增体系构成的检测试剂盒。本发明所述多重扩增体系通过一次扩增即可获得大量信息；扩增产物带有荧光，可直接在测序仪上进行检测，结果准确，重复性高，数据可建立标准参考数据库；操作简便，需时短，易于规模化及自动化。