Method for producing avian influenza vaccine by adopting MDCK cell line and product of avian influenza vaccine

• Main Authors: ZHANG XIAONAN, ZHAN XUANZI, SHI WEISONG, ZHENG CAILI, LU SHAOHUA, KONG FENGYING, LIU DECHENG
• Format: Patent
• Language: Chinese; English
• Published: 29.09.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to a method for producing an avian influenza vaccine by adopting an MDCK cell line and a product of the avian influenza vaccine. The method comprises the following steps of domesticating adherent MDCK cells into the stably proliferated serum-free full-suspension culture type MDCK cell line, then performing toxin inoculation and proliferation, separating and collecting virus liquid, performing inactivation, and adding an adjuvant to prepare the vaccine. The cell line is used for replacing chick embryo tissue culture to prepare avian influenza virus and the avian influenzavaccine, so that the problem that chick embryos are polluted by exogenous virus can be solved; and by strictly controlling raw materials and culture conditions, the purity of the produced vaccine is ensured, the safety of vaccine is ensured, the method can be applied to large-scale production under safe and economic conditions, and the effective avian influenza vaccine with high purity and ideal industrial yield is obtain