Endoglucanase mutant, gene, engineering bacterium and application thereof
The invention discloses an endoglucanase mutant, a genetically engineered bacterium and application of the endoglucanase mutant in degradation of cellulose. The endoglucanase mutant is obtained by mutating lysine at the 202 position of an amino acid sequence shown in SEQ ID No. 3 into arginine. The...
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Main Authors | , , , , , , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
22.09.2020
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses an endoglucanase mutant, a genetically engineered bacterium and application of the endoglucanase mutant in degradation of cellulose. The endoglucanase mutant is obtained by mutating lysine at the 202 position of an amino acid sequence shown in SEQ ID No. 3 into arginine. The specific activity of the endoglucanase mutant disclosed is obviously improved by about 1.4 times compared with that of a wild enzyme. When microcrystalline cellulose is used as a substrate; through the common reaction of cellulase and mutant enzyme, the reaction catalytic efficiency is remarkably improved by 23.5% compared with that of single degradation of a substrate with cellulase, it can be seen that the mutant enzyme remarkably improves the enzyme activity, the economic cost of industrial application can be reduced, and an application basis is provided for the mutant enzyme in the field of industrial production of food bioethanol and the like.
本发明公开一种内切葡聚糖苷酶突变体、基因工程菌及其在降解纤维素中的应用。所述内切葡聚糖苷酶突变体是将SEQ ID No.3所示氨基酸序 |
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Bibliography: | Application Number: CN202010471544 |