Mutant and genetically modified bacillus cells and methods thereof for increased protein production

• Main Authors: DEL CHASE SHANNON, MASSON HELEN OLIVIA, FIORESI CAROL MARIE, VAN KIMMENADE ANITA, FRISCH RYAN L
• Format: Patent
• Language: Chinese; English
• Published: 15.09.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The instant disclosure is generally related to novel Bacillus sp. mutants capable of producing increased amounts of industrially relevant proteins of interest. Certain embodiments of the disclosure are related to modified Bacillus sp. cells comprising an introduced polynucleotide encoding a variant GlcT protein. Other embodiments are related to methods and compositions for producing endogenous and/or heterologous proteins of interest in the modified Bacillus sp. (daughter) cells, whereas certain other embodiments are directed to nucleic acid sequences, particularly polynucleotide open readingframe (ORF) sequences, vectors thereof and DNA expression constructs thereof, encoding variant GlcT proteins of the disclosure. 本公开总体上涉及能够产生增加量的工业相关目的蛋白的新颖的芽孢杆菌属物种突变体。本公开的某些实施例涉及经修饰的芽孢杆菌属物种细胞，所述经修饰的芽孢杆菌属物种细胞包含引入的编码变体GlcT蛋白质的多核苷酸。其他实施例涉及在经修饰的芽孢杆菌属物种(子代)细胞中产生内源和/或异源目的蛋白的方法和组合物，而某些其他实施例涉及编码本公开的变体GlcT蛋白质的核酸序列，特别是多核苷酸可读框(ORF)序列，其载体及其DNA表达构建体。