Staphylococcus aureus transposon sequencing library and construction method thereof

The invention discloses a staphylococcus aureus transposon sequencing library and a construction method thereof. The construction method comprises the following steps: (1) constructing plasmid: firstly constructing a plasmid skeleton and a transposon, and then synthesizing a complete pSATn21 plasmid...

Full description

Saved in:
Bibliographic Details
Main Authors ZHAN JIAHUAN, CHEN KAISEN, CHEN QIANG, LUO DONG
Format Patent
LanguageChinese
English
Published 15.09.2020
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMBINATORIAL CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:The invention discloses a staphylococcus aureus transposon sequencing library and a construction method thereof. The construction method comprises the following steps: (1) constructing plasmid: firstly constructing a plasmid skeleton and a transposon, and then synthesizing a complete pSATn21 plasmid; (2) transforming the plasmid: transferring the pSATn21 plasmid into DC10B for modification, and then transferring the pSATn21 plasmid into a staphylococcus aureus Newman standard strain; (3) performing induction transposition: performing culture medium culture, transposition and elution on the strain obtained in the previous step to obtain a library; and (4) verifying the library: cutting a certain number of nucleotide sequences at the downstream of the mme I restriction enzyme cutting site toobtain a fusion fragment with a transposon fragment and a certain number of fusion fragments from a staphylococcus aureus Newman genome, and carrying out high-throughput sequencing in a linker-addedPCR enrichment mode. The c
Bibliography:Application Number: CN202010648075