AUGMENTATION OF FIBROBLAST REGENERATIVE ACTIVITY

• Main Authors: O'HEERON PETE, ICHIM THOMAS
• Format: Patent
• Language: Chinese; English
• Published: 04.09.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

Embodiments of the disclosure include methods and compositions for use of prepared fibroblasts and/or conditioned media therefrom. In particular embodiments, the prepared fibroblasts have enhanced regenerative and may have an increase in secretion of one or more cytokines and/or growth factors; may have an increase in anti-apoptotic activity; and/or may have a modulated immunogenicity. In specificembodiments, the fibroblasts are prepared by exposure to hypoxia and/or carbon monoxide. 本公开内容的实施方案包括使用制备的成纤维细胞和/或来自其的条件培养基的方法和组合物。在特定实施方案中，所制备的成纤维细胞具有增强的再生能力并且可以具有一种或多种细胞因子和/或生长因子的分泌增加；可以具有抗凋亡活性的增加；和/或可以具有调节的免疫原性。在具体的实施方案中，通过暴露于低氧和/或一氧化碳来制备成纤维细胞。