Method and kit for detecting CYP2C19 genotyping based on real-time fluorescent quantitative PCR

• Main Authors: ZHOU QIHAO, SONG DONGLIANG, LI JIANHUI, YAN GUANGHAO, SUN YONGXIANG
• Format: Patent
• Language: Chinese; English
• Published: 01.09.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for detecting CYP2C19 genotyping based on real-time fluorescent quantitative PCR (Polymerase Chain Reaction) for non-disease treatment and diagnosis purposes, comprising the following steps of: 1, performing fluorescent quantitative PCR amplification by taking DNA (Deoxyribonucleic Acid) of a sample to be detected as a template to obtain a PCR amplification product; 2, detecting a fluorescence signal of an amplification product; 3, judging whether a sample contains the CYP2C19 * 2 genotype or not, according to a detection result, wherein a reaction system for PCR amplification contains a specific primer pair for amplifying the CYP2C19 * 2 gene and a fluorescent probe for detecting the CYP2C19 * 2 gene. The invention also discloses a primer pair and a fluorescent probe for detecting the CYP2C19 genotyping. The method provided by the invention has high specificity and high detection result accuracy. 本发明公开了一种非疾病治疗和诊断目的的基于实时荧光定量PCR的检测CYP2C19基因分型的方法，包括如下步骤：第一步，以待测样品的DNA为模板，进行荧光定量PC