Rapid screening method and kit of novel coronavirus based on thermostatic nucleic acid amplification

• Main Authors: FU WEILING, XIA KE, ZHANG YANG
• Format: Patent
• Language: Chinese; English
• Published: 25.08.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses a rapid screening method and a kit of novel coronavirus based on thermostatic nucleic acid amplification and specifically relates to the field of virus detection. The method includes the following steps: adopting gold magnetic nanoparticles as carriers and utilizing a sold-phase rolling circle amplification technology to construct a thermostatic nucleic acid amplification system of the coronavirus; and achieving specific capture through the reaction between amplification products and a detection probe containing paper microfluidic chip so as to realize colloidal gold agglutination and specific chromogenic reaction. According to the invention, through replacing traditional PCR by the thermostatic amplification and combining a paper microfluidic immunochromatography thechnology, the key thermostatic amplification technology and the supporting kit of 2019-nCov virus are constructed; through systematically combining the paper microfluidic chip with the thermostaticnucleic acid amplificatio