培养细胞的制造方法、脊髓损伤疾病的治疗剂的制造方法

• Format: Patent
• Language: Chinese
• Published: 26.03.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

本发明的课题是提供一种细胞培养方法，其不采用由病毒所引起的基因导入法，而进行安全的GDNF基因表现(mRNA)的增加。本发明的解决手段是一种培养细胞的制造方法，其包含：培养工序，在包含SAG、嘌吗啡胺(Purmorphamine)以及音猬因子(Sonic hedgehog，SHH)蛋白质的任一种或二种以上的无血清培养基中培养人类皮肤来源干细胞；无血清培养基可更包含B-27添加剂、ROCK抑制剂、EGF、FGF2。 [Problem to Be Solved] The present invention is to provide a method of culturing a cell, which method safely increases GDNF mRNA expression without introducing a gene with a virus.[Means to Solve the Problem] A method of producing a cultured cell comprising glial cell line-derived neurotrophic factor (GDNF) mRNA, comprising the step of culturing a human skin-derived stem cell in a serum-free medium comprising at least one of SAG, purmorphamine and sonic hedgehog (SHH) protein. The medium may further comprise B-27 supplement, a ROCK inhibitor, EGF and FGF2.