USE OF NUCLEIC ACID MOLECULES IN PLANT TRANSGENATION, MOLECULAR BREEDING, DISEASE CONTROL AND MOLECULAR MARKERS

• Main Authors: KONG LINGRANG, WANG HONGWEI, GE WENYANG, SUN SILONG
• Format: Patent
• Language: Chinese; English
• Published: 18.08.2020
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses application of nucleic acid molecules in plant transgenosis, molecular breeding, disease control and molecular markers. According to the invention, nucleic acid molecules for coding the deepoxidation protease are expressed in plants through genetic engineering, so that epoxy groups of trichothecene toxins are efficiently removed, the endotoxin content of plants is directlyreduced. Meanwhile, the disease resistance of crops can be improved, and the quality of the crops is remarkably improved. According to the invention, the blank that no clear gene can be used for detoxifying trichothecene toxins by removing epoxy groups in the prior art is filled, and the trichothecene toxins have a wide application prospect in the aspects of plant transgenosis, molecular breedingand disease prevention and treatment. 本发明公开核酸分子在植物转基因、分子育种、病害防治和分子标记物中的用途。本发明通过基因工程使编码去环氧基蛋白酶的核酸分子在植物内表达，从而高效去除单端孢霉烯族毒素的环氧基，直接减少植物体内毒素含量，同时还可提高作物的抗病能力，显著提高作物品质。本发明弥补了目前尚未有明确的基因可通过去环氧基催化单端孢霉烯族毒素进行解毒的空白，在植物转基因、分子育种、病害防治方面具有广阔的应用